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ProductsBack/anti-OSTF1 antibody [9G4]

anti-OSTF1 antibody [9G4]

ARG56974
Arigo Biolaboratories
ApplicationsFlow Cytometry, Western Blot
Product group Antibodies
ReactivityHuman
TargetOSTF1
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Overview

  • Supplier
    Arigo Biolaboratories
  • Product Name
    anti-OSTF1 antibody [9G4]
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    9G4
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID26578
  • Target name
    OSTF1
  • Target description
    osteoclast stimulating factor 1
  • Target synonyms
    OSF, SH3P2, bA235O14.1, osteoclast-stimulating factor 1
  • Host
    Mouse
  • Isotype
    IgG2a
  • Scientific Description
    Osteoclast-stimulating factor-1 is an intracellular protein produced by osteoclasts that indirectly induces osteoclast formation and bone resorption (Reddy et al., 1998 [PubMed 10092216]).[supplied by OMIM, Mar 2008]
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of OSTF1 using anti-OSTF1 antibody (A05988-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human placenta tissue lysates, Lane 5: rat liver tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OSTF1 antigen affinity purified polyclonal antibody (Catalog # A05988-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OSTF1 at approximately 28 kDa. The expected band size for OSTF1 is at 24,28 kDa.
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