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Anti-OTUB1 Antibody

144-10313
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetOTUB1
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-OTUB1 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55611
  • Target name
    OTUB1
  • Target description
    OTU deubiquitinase, ubiquitin aldehyde binding 1
  • Target synonyms
    HSPC263, OTB1, OTU1, ubiquitin thioesterase OTUB1, OTU domain, ubiquitin aldehyde binding 1, OTU domain-containing ubiquitin aldehyde-binding protein 1, OTU-domain Ubal-binding 1, deubiquitinating enzyme OTUB1, otubain-1, ubiquitin-specific protease otubain 1, ubiquitin-specific-processing protease OTUB1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96FW1
  • Protein Name
    Ubiquitin thioesterase OTUB1
  • Scientific Description
    OTUB1 Polyclonal Antibody
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of OTUB1 using anti-OTUB1 antibody (A04361-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OTUB1 antigen affinity purified polyclonal antibody (Catalog # A04361-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OTUB1 at approximately 36 kDa. The expected band size for OTUB1 is at 31 kDa.
Product group Antibodies
Boster Bio
Anti-OTUB1 Antibody Picoband(r)A04361-1-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetOTUB1
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Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with OTUB1 antibody [N1C1] (GTX101973) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Product group Antibodies
GeneTex
OTUB1 antibody [N1C1]GTX101973
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
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TargetOTUB1
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Anti-OTUB1 AntibodyHPA039176
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ReactivityHuman, Mouse, Rat
TargetOTUB1
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Western blot All lanes: OTUB1 antibody at 2microg/ml Lane 1: EC109 whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 32, 36 kDa Observed band size: 80 kDa
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TargetOTUB1
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