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Immunohistochemical staining of human cerebral cortex shows moderate nuclear positivity in glial cells.
Immunohistochemical staining of human cerebral cortex shows moderate nuclear positivity in glial cells.
Immunohistochemical staining of human cerebral cortex shows moderate nuclear positivity in glial cells.

Anti-OTUB1 Antibody

HPA039176
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetOTUB1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-OTUB1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55611
  • Target name
    OTUB1
  • Target description
    OTU deubiquitinase, ubiquitin aldehyde binding 1
  • Target synonyms
    deubiquitinating enzyme OTUB1; HSPC263; OTB1; OTU domain, ubiquitin aldehyde binding 1; OTU domain-containing ubiquitin aldehyde-binding protein 1; OTU1; otubain-1; OTU-domain Ubal-binding 1; ubiquitin thioesterase OTUB1; ubiquitin-specific protease otubain 1; ubiquitin-specific-processing protease OTUB1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96FW1
  • Protein Name
    Ubiquitin thioesterase OTUB1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Western blot All lanes: OTUB1 antibody at 2microg/ml Lane 1: EC109 whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 32, 36 kDa Observed band size: 80 kDa
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Figure 1. Western blot analysis of OTUB1 using anti-OTUB1 antibody (A04361-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OTUB1 antigen affinity purified polyclonal antibody (Catalog # A04361-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OTUB1 at approximately 36 kDa. The expected band size for OTUB1 is at 31 kDa.
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