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Immunohistochemical staining of human colon shows strong cytoplasmic and nuclear positivity in glandular cells.
Immunohistochemical staining of human colon shows strong cytoplasmic and nuclear positivity in glandular cells.
Immunohistochemical staining of human colon shows strong cytoplasmic and nuclear positivity in glandular cells.

Anti-OTUD6B Antibody

HPA024503
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetOTUD6B
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-OTUD6B
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51633
  • Target name
    OTUD6B
  • Target description
    OTU deubiquitinase 6B
  • Target synonyms
    CGI-77; deubiquitinase OTUD6B; DUBA5; DUBA-5; IDDFSDA; OTU domain containing 6B; OTU domain-containing protein 6B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8N6M0
  • Protein Name
    Deubiquitinase OTUD6B
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of OTUD6B using anti-OTUD6B antibody (A15219). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OTUD6B antigen affinity purified polyclonal antibody (A15219) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OTUD6B at approximately 36 kDa. The expected band size for OTUD6B is at 34 kDa.
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