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OTUD6B Antibody

LS-C749513
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetOTUD6B
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    OTUD6B Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 21kDa/33kDa, while the observed MW by Western blot was 38kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 21kDa/33kDa, while the observed MW by Western blot was 38kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID51633
  • Target name
    OTUD6B
  • Target description
    OTU deubiquitinase 6B
  • Target synonyms
    CGI-77, DUBA-5, DUBA5, IDDFSDA, deubiquitinase OTUD6B, OTU domain containing 6B, OTU domain-containing protein 6B
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of OTUD6B using anti-OTUD6B antibody (A15219). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OTUD6B antigen affinity purified polyclonal antibody (A15219) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OTUD6B at approximately 36 kDa. The expected band size for OTUD6B is at 34 kDa.
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Western blot All lanes: OTUD6B antibody at 4microg/ml Lane 1: PC-3 whole cell lysate Lane 2: 293T whole cell lysate Lane 3: MDA-MB-231 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 34, 22 kDa Observed band size: 34 kDa
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