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Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.

Anti-OXER1 Antibody

HPA051433
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
TargetOXER1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-OXER1
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID165140
  • Target name
    OXER1
  • Target description
    oxoeicosanoid receptor 1
  • Target synonyms
    5-oxo-ETE acid G-protein-coupled receptor 1; 5-oxo-ETE G-protein coupled receptor; GPCR; GPR170; G-protein coupled receptor 170; G-protein coupled receptor R527; G-protein coupled receptor TG1019; OXE receptor; oxoeicosanoid (OXE) receptor 1; oxoeicosanoid receptor 1; TG1019
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8TDS5
  • Protein Name
    Oxoeicosanoid receptor 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of OXER1 using anti-OXER1 antibody (A10420-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OXER1 antigen affinity purified polyclonal antibody (Catalog # A10420-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OXER1 at approximately 43-46 kDa. The expected band size for OXER1 is at 43-46 kDa.
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