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ProductsBack/OXER1 Antibody
Western Blot analysis of NIH-3T3 cells using TG1019 Polyclonal Antibody
Western Blot analysis of NIH-3T3 cells using TG1019 Polyclonal Antibody
Western Blot analysis of NIH-3T3 cells using TG1019 Polyclonal Antibody

OXER1 Antibody

CSB-PA004271
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetOXER1
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Overview

  • Supplier
    Cusabio
  • Product Name
    OXER1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID165140
  • Target name
    OXER1
  • Target description
    oxoeicosanoid receptor 1
  • Target synonyms
    5-oxo-ETE acid G-protein-coupled receptor 1; 5-oxo-ETE G-protein coupled receptor; GPCR; GPR170; G-protein coupled receptor 170; G-protein coupled receptor R527; G-protein coupled receptor TG1019; OXE receptor; oxoeicosanoid (OXE) receptor 1; oxoeicosanoid receptor 1; TG1019
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8TDS5
  • Protein Name
    Oxoeicosanoid receptor 1
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of OXER1 using anti-OXER1 antibody (A10420-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OXER1 antigen affinity purified polyclonal antibody (Catalog # A10420-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OXER1 at approximately 43-46 kDa. The expected band size for OXER1 is at 43-46 kDa.
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