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Anti-P-Cadherin [6A9]

AB02535-1.1
Absolute Antibody
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetCDH3
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-P-Cadherin [6A9]
  • Delivery Days Customer
    9
  • Application Supplier Note
    The antibody detected human P-Cadherin from A431 cell lysate by Western blot analysis. The antibody was used to immunoprecipitate P-Cadherin. Further, the antibody was shown to have inhibitory activity, by disrupting P-cadherin-mediated cell-cell adhesion with A-431 cells (Lewis et al., 1994; PMID: 8006450). The antibody detected P-cadherin by immunofluorescence (Owens et al., 2000; PMID: 10708559).
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    6A9
  • Gene ID1001
  • Target name
    CDH3
  • Target description
    cadherin 3
  • Target synonyms
    cadherin 3, type 1, P-cadherin (placental); cadherin-3; calcium-dependent adhesion protein, placental; CDHP; HJMD; PCAD
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP22223
  • Protein Name
    Cadherin-3
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of P cadherin/CDH3 using anti-P cadherin/CDH3 antibody (A03353-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P cadherin/CDH3 antigen affinity purified polyclonal antibody (Catalog # A03353-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for P cadherin/CDH3 at approximately 120 kDa. The expected band size for P cadherin/CDH3 is at 91 kDa.
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