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Anti-p21WAF1 (Center H450) Antibody

102-20929
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetLCMT2
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-p21WAF1 (Center H450) Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    RB19096
  • Gene ID9836
  • Target name
    LCMT2
  • Target description
    leucine carboxyl methyltransferase 2
  • Target synonyms
    p21WAF1/CIP1 promoter-interacting protein; PPM2; tRNA wybutosine-synthesizing protein 4; tRNA(Phe) (7-(3-amino-3-(methoxycarbonyl)propyl)wyosine(37)-N)-methoxycarbonyltransferase; tRNA(Phe) (7-(3-amino-3-carboxypropyl)wyosine(37)-O)-methyltransferase; tRNA-Wybutosine-synthesizing protein 4; tRNA-yW synthesizing protein 4; TYW4
  • Host
    Rabbit
  • Protein IDO60294
  • Protein Name
    tRNA wybutosine-synthesizing protein 4
  • Scientific Description
    Rabbit Anti-p21WAF1 (Center H450) Antibody, 400 microl
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LCMT2 using anti-LCMT2 antibody (A14797-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hacat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LCMT2 antigen affinity purified polyclonal antibody (Catalog # A14797-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LCMT2 at approximately 76 kDa. The expected band size for LCMT2 is at 76 kDa.
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