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Anti-P70 S6K Antibody

144-64571
RayBiotech
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetRPS6KB1
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-P70 S6K Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Conjugate
    Unconjugated
  • Gene ID6198
  • Target name
    RPS6KB1
  • Target description
    ribosomal protein S6 kinase B1
  • Target synonyms
    PS6K, S6K, S6K-beta-1, S6K1, STK14A, p70 S6KA, p70(S6K)-alpha, p70-S6K, p70-alpha, ribosomal protein S6 kinase beta-1, ribosomal protein S6 kinase I, ribosomal protein S6 kinase, 70kDa, polypeptide 1, serine/threonine kinase 14 alpha, serine/threonine-protein kinase 14A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP23443
  • Protein Name
    Ribosomal protein S6 kinase beta-1
  • Scientific Description
    P70 S6K pAb
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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This image was generously provided by Piotr Mamczur at University of Wroclaw as part of the Bioss Discovery Program. Mouse squamous cell carcinoma (KLN-205 cell line) were probed with Rabbit Anti-RPS6KB1 Polyclonal Antibody (bs-1426R) at 1:100 followed by at FITC conjugated secondary antibody and DAPI staining
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Figure 1. Western blot analysis of S6K1/RPS6KB1 using anti-S6K1/RPS6KB1 antibody (A01475-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT4 whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: human 293T whole cell lysates, Lane 6: human placenta tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-S6K1/RPS6KB1 antigen affinity purified polyclonal antibody (Catalog # A01475-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for S6K1/RPS6KB1 at approximately 59 kDa. The expected band size for S6K1/RPS6KB1 is at 59 kDa.
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