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Immunohistochemical staining of human Cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human Cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human Cerebral cortex shows strong cytoplasmic positivity in neuronal cells.

Anti-PA2G4 Antibody

HPA016484
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetPA2G4
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PA2G4
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5036
  • Target name
    PA2G4
  • Target description
    proliferation-associated 2G4
  • Target synonyms
    cell cycle protein p38-2G4 homolog; EBP1; ErbB-3 binding protein 1; ErbB-3 receptor binding protein; erbB3-binding protein 1; ErbB3-binding protein Ebp1; HG4-1; p38-2G4; proliferation-associated 2G4, 38kD; proliferation-associated 2G4, 38kDa; proliferation-associated protein 2G4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UQ80
  • Protein Name
    Proliferation-associated protein 2G4
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of EBP1 using anti-EBP1 antibody (A02791). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: rat liver tissue lysates, lane 2: NIH3T3 whole cell lysates, lane 3: HEPG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EBP1 antigen affinity purified polyclonal antibody (Catalog # A02791) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EBP1 at approximately 43KD. The expected band size for EBP1 is at 43KD.
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