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PA2G4 / EBP1 Antibody

LS-C831018
LifeSpan BioSciences
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetPA2G4
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PA2G4 / EBP1 Antibody
  • Delivery Days Customer
    23
  • Applications
    ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA (1:5000 - 1:10000), IHC (1:50 - 1:200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5036
  • Target name
    PA2G4
  • Target description
    proliferation-associated 2G4
  • Target synonyms
    cell cycle protein p38-2G4 homolog; EBP1; ErbB-3 binding protein 1; ErbB-3 receptor binding protein; erbB3-binding protein 1; ErbB3-binding protein Ebp1; HG4-1; p38-2G4; proliferation-associated 2G4, 38kD; proliferation-associated 2G4, 38kDa; proliferation-associated protein 2G4
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of EBP1 using anti-EBP1 antibody (A02791). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: rat liver tissue lysates, lane 2: NIH3T3 whole cell lysates, lane 3: HEPG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EBP1 antigen affinity purified polyclonal antibody (Catalog # A02791) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EBP1 at approximately 43KD. The expected band size for EBP1 is at 43KD.
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