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Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular cells.

Anti-PAFAH1B2 Antibody

HPA051836
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPAFAH1B2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PAFAH1B2 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5049
  • Target name
    PAFAH1B2
  • Target description
    platelet activating factor acetylhydrolase 1b catalytic subunit 2
  • Target synonyms
    HEL-S-303, platelet-activating factor acetylhydrolase IB subunit alpha2, PAF acetylhydrolase 30 kDa subunit, PAF-AH 30 kDa subunit, PAF-AH subunit beta, PAF-AH1b alpha 2 subunit, PAFAH subunit beta, epididymis secretory protein Li 303, epididymis secretory sperm binding protein, intracellular platelet-activating factor acetylhydrolase alpha 2 subunit, platelet-activating factor acetylhydrolase 1b, catalytic subunit 2 (30kDa), platelet-activating factor acetylhydrolase IB subunit beta
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP68402
  • Protein Name
    Platelet-activating factor acetylhydrolase IB subunit alpha2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of PAFAH1B2 using anti-PAFAH1B2 antibody (A07067). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAFAH1B2 antigen affinity purified polyclonal antibody (Catalog # A07067) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAFAH1B2 at approximately 26 kDa. The expected band size for PAFAH1B2 is at 26 kDa.
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