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Immunofluorescent analysis of MCF-7 cells using CSB-PA017384LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunofluorescent analysis of MCF-7 cells using CSB-PA017384LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunofluorescent analysis of MCF-7 cells using CSB-PA017384LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

PAFAH1B2 Antibody

CSB-PA017384LA01HU
Cusabio
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPAFAH1B2
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Overview

  • Supplier
    Cusabio
  • Product Name
    PAFAH1B2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5049
  • Target name
    PAFAH1B2
  • Target description
    platelet activating factor acetylhydrolase 1b catalytic subunit 2
  • Target synonyms
    HEL-S-303, platelet-activating factor acetylhydrolase IB subunit alpha2, PAF acetylhydrolase 30 kDa subunit, PAF-AH 30 kDa subunit, PAF-AH subunit beta, PAF-AH1b alpha 2 subunit, PAFAH subunit beta, epididymis secretory protein Li 303, epididymis secretory sperm binding protein, intracellular platelet-activating factor acetylhydrolase alpha 2 subunit, platelet-activating factor acetylhydrolase 1b, catalytic subunit 2 (30kDa), platelet-activating factor acetylhydrolase IB subunit beta
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP68402
  • Protein Name
    Platelet-activating factor acetylhydrolase IB subunit alpha2
  • Scientific Description
    Inactivates PAF by removing the acetyl group at the sn-2 position. This is a catalytic subunit.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PAFAH1B2 using anti-PAFAH1B2 antibody (A07067). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAFAH1B2 antigen affinity purified polyclonal antibody (Catalog # A07067) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAFAH1B2 at approximately 26 kDa. The expected band size for PAFAH1B2 is at 26 kDa.
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