More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Anti-pAKT [AbAb-pAKT]

Anti-pAKT [AbAb-pAKT]

AB04102-10.0-BT
Absolute Antibody
ApplicationsImmunoPrecipitation, Western Blot, ELISA
Product group Antibodies
TargetAKT1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-pAKT [AbAb-pAKT]
  • Delivery Days Customer
    9
  • Application Supplier Note
    The binding reactivity of this antibody to recombinant phosphorylated protein kinase B peptide was confirmed using ELISA. The binding reactivity was further confirmed using western blot, of pAkt from NIH3T3 cell lysates. The scFv version of this antibody was used fused to human IgG1 using a suitable linker for the generation of scFv-Fc mini-antibody. This minibody was capable of immunoprecipitating pAkt proteins. (US20090233358).
  • Applications
    ImmunoPrecipitation, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    AbAb-pAKT
  • Gene ID207
  • Target name
    AKT1
  • Target description
    AKT serine/threonine kinase 1
  • Target synonyms
    AKT; AKT1m; PKB; PKB alpha; PKB-ALPHA; PRKBA; protein kinase B alpha; proto-oncogene c-Akt; RAC; rac protein kinase alpha; RAC-ALPHA; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha; serine-threonine protein kinase; v-akt murine thymoma viral oncogene homolog 1; v-akt murine thymoma viral oncogene-like protein 1
  • Host
    Human
  • Isotype
    IgG1
  • Protein IDP31749
  • Protein Name
    RAC-alpha serine/threonine-protein kinase
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
RayBiotech
Anti-AKT1 Antibody144-11016
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
TargetAKT1
  • SizePrice
Product group Antibodies
Assay Genie
Phospho-AKT1-S308+AKT2-S309+AKT3-S305 AntibodyCABP1266
TargetAKT1
  • SizePrice
Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-Phospho-Akt (Ser473) RM251 at a 1:400 dilution.
Product group Antibodies
RevMAb Biosciences
anti-Phospho-Akt (Ser473) (human), Rabbit Monoclonal (RM251)REV-31-1131-00
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetAKT1
  • SizePrice
Product group Antibodies
Everest Biotech
Goat anti-AKT1EB06875
ApplicationsWestern Blot, ELISA
TargetAKT1
  • SizePrice
Figure 1. Western blot analysis of AKT1,2,3 using anti-AKT1,2,3 antibody (A00024-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKT1,2,3 antigen affinity purified polyclonal antibody (Catalog # A00024-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKT1,2,3 at approximately 60 kDa. The expected band size for AKT1,2,3 is at 60 kDa.
Product group Antibodies
Boster Bio
Anti-AKT1,2,3 Antibody Picoband(r)A00024-2-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
TargetAKT1
  • SizePrice
Product group Antibodies
References
GeneTex
AKT1 antibodyGTX110613
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetAKT1
  • SizePrice
Product group Antibodies
Applied Biological Materials
Anti-AKT1Y058027
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
  • SizePrice
Product group Antibodies
MedChem Express
AKT1 Antibody (YA834, Mouse)HY-P80007
TargetAKT1
  • SizePrice
Product group Antibodies
Biomatik
Akt1 Polyclonal AntibodyCAC07008
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
TargetAKT1
  • SizePrice
Western Blot analysis of A549 cells using Akt Polyclonal Antibody
Product group Antibodies
Cusabio
AKT1/AKT2/AKT3 AntibodyCSB-PA000848
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice