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Immunohistochemical staining of human breast shows strong cytoplasmic and membranous positivity in glandular cells.
Immunohistochemical staining of human breast shows strong cytoplasmic and membranous positivity in glandular cells.
Immunohistochemical staining of human breast shows strong cytoplasmic and membranous positivity in glandular cells.

Anti-PALMD Antibody

HPA030549
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetPALMD
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PALMD
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID54873
  • Target name
    PALMD
  • Target description
    palmdelphin
  • Target synonyms
    C1orf11; palmdelphin; PALML; paralemmin-like protein; paralemnin-like protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NP74
  • Protein Name
    Palmdelphin
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PALMD using anti-PALMD antibody (A11148-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PALMD antigen affinity purified polyclonal antibody (Catalog # A11148-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PALMD at approximately 68 kDa. The expected band size for PALMD is at 63 kDa.
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