Anti-PAR2 [Ab4999 (Par-B)]

Absolute Antibody

Anti-PAR2 [Ab4999 (Par-B)]

7

This antibody was generated by the affinity maturation of a parental clone Ab3777. This antibody exhibited concentration-dependent binding to human PAR-2 transfected HEK293 cells with EC50 values of 0.2 nM. ELISA experiments demonstrated specific binding of this antibody to PAR-2 peptide (coupled to Transferrin as carrier). This antibody did not show any binding affinity for PAR-4 peptide or PAR-1 peptide. This antibody showed potent inhibition of calcium release in the human lung epithelial cell line A549 stimulated with trypsin similar to the parental clone Ab3777. (US20110311553).

ELISA

ELISA

Research Use Only

Monoclonal

Ab4999 (Par-B)

F2RL1

F2R like trypsin receptor 1

GPR11, PAR2, proteinase-activated receptor 2, G-protein coupled receptor 11, coagulation factor II (thrombin) receptor-like 1, protease-activated receptor 2, thrombin receptor-like 1

Mouse

IgG1

Proteinase-activated receptor 2

This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG4 format for improved compatibility with existing reagents assays and techniques.

Human, Monkey, Mouse, Rat

Human, mouse, rat, cynomolgus monkey

The original antibody was generated using phage display techniques to pan for antibodies (Fab fragments) capable of immunospecifically binding to residues spanning the protease cleavage site at the N-terminal of PAR-2. The affinity maturation of the selected parental clone Ab3777 resulted in generation of the antibody.

-20°C,2°C to 8°C

12352203