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ProductsBack/F2RL1 / PAR2 Antibody

F2RL1 / PAR2 Antibody

LS-C410329
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetF2RL1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    F2RL1 / PAR2 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 44kDa, while the observed MW by Western blot was 44kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:200 - 1:1000) The predicted MW is 44kDa, while the observed MW by Western blot was 44kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID2150
  • Target name
    F2RL1
  • Target description
    F2R like trypsin receptor 1
  • Target synonyms
    GPR11, PAR2, proteinase-activated receptor 2, G-protein coupled receptor 11, coagulation factor II (thrombin) receptor-like 1, protease-activated receptor 2, thrombin receptor-like 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PAR2 using anti-PAR2 antibody (PB9700). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40ug of sample under reducing conditions. Lane 1: HELA Whole Cell Lysate, Lane 2: COLO320 Whole Cell Lysate, Lane 3: SW620 Whole Cell Lysate, Lane 4: HEPG2 Whole Cell Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAR2 antigen affinity purified polyclonal antibody (Catalog # PB9700) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAR2 at approximately 49 kDa. The expected band size for PAR2 is at 44 kDa.
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