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Immunohistochemical staining of human skin shows weak to moderate cytoplasmic positivity in epidermal cells.
Immunohistochemical staining of human skin shows weak to moderate cytoplasmic positivity in epidermal cells.
Immunohistochemical staining of human skin shows weak to moderate cytoplasmic positivity in epidermal cells.

Anti-PARD3 Antibody

HPA030443
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPARD3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PARD3 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID56288
  • Target name
    PARD3
  • Target description
    par-3 family cell polarity regulator
  • Target synonyms
    ASIP, Baz, PAR3, PAR3alpha, PARD-3, PARD3A, PPP1R118, SE2-5L16, SE2-5LT1, SE2-5T2, partitioning defective 3 homolog, CTCL tumor antigen se2-5, PAR3-alpha, atypical PKC isotype-specific interacting protein, bazooka, par-3 family cell polarity regulator alpha, par-3 partitioning defective 3 homolog, protein phosphatase 1, regulatory subunit 118
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8TEW0
  • Protein Name
    Partitioning defective 3 homolog
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of PAR-3/PARD3 using anti-PAR-3/PARD3 antibody (A01897-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human CACO-2 whole cell lysates, Lane 5: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAR-3/PARD3 antigen affinity purified polyclonal antibody (Catalog # A01897-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAR-3/PARD3 at approximately 151 kDa. The expected band size for PAR-3/PARD3 is at 151 kDa.
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