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Formalin-fixed and paraffin embedded human cervical cancer labeled with Anti-PAR3 Polyclonal Antibody, Unconjugated (bs-2921R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human cervical cancer labeled with Anti-PAR3 Polyclonal Antibody, Unconjugated (bs-2921R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human cervical cancer labeled with Anti-PAR3 Polyclonal Antibody, Unconjugated (bs-2921R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

PAR3 Polyclonal Antibody

BS-2921R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPARD3
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Overview

  • Supplier
    Bioss
  • Product Name
    PAR3 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID56288
  • Target name
    PARD3
  • Target description
    par-3 family cell polarity regulator
  • Target synonyms
    ASIP, Baz, PAR3, PAR3alpha, PARD-3, PARD3A, PPP1R118, SE2-5L16, SE2-5LT1, SE2-5T2, partitioning defective 3 homolog, CTCL tumor antigen se2-5, PAR3-alpha, atypical PKC isotype-specific interacting protein, bazooka, par-3 family cell polarity regulator alpha, par-3 partitioning defective 3 homolog, protein phosphatase 1, regulatory subunit 118
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8TEW0
  • Protein Name
    Partitioning defective 3 homolog
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of PAR-3/PARD3 using anti-PAR-3/PARD3 antibody (A01897-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human CACO-2 whole cell lysates, Lane 5: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAR-3/PARD3 antigen affinity purified polyclonal antibody (Catalog # A01897-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAR-3/PARD3 at approximately 151 kDa. The expected band size for PAR-3/PARD3 is at 151 kDa.
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