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Immunohistochemical staining of human urinary bladder shows moderate positivity in nucleoli in urothelial cells.
Immunohistochemical staining of human urinary bladder shows moderate positivity in nucleoli in urothelial cells.
Immunohistochemical staining of human urinary bladder shows moderate positivity in nucleoli in urothelial cells.

Anti-PARP3 Antibody

HPA067657
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPARP3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PARP3 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10039
  • Target name
    PARP3
  • Target description
    poly(ADP-ribose) polymerase family member 3
  • Target synonyms
    ADPRT3, ADPRTL2, ADPRTL3, ARTD3, IRT1, PADPRT-3, protein mono-ADP-ribosyltransferase PARP3, ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase)-like 2, ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase)-like 3, ADP-ribosyltransferase diphtheria toxin-like 3, ADPRT-3, DNA ADP-ribosyltransferase PARP3, NAD(+) ADP-ribosyltransferase 3, NAD+ ADP-ribosyltransferase 3, poly [ADP-ribose] polymerase 3, poly(ADP-ribose) synthetase-3, poly[ADP-ribose] synthase 3, poly[ADP-ribose] synthetase 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y6F1
  • Protein Name
    Protein mono-ADP-ribosyltransferase PARP3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of PARP3 using anti-PARP3 antibody (A03762-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: monkey COS-7 whole cell lysates, Lane 2: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PARP3 antigen affinity purified polyclonal antibody (Catalog # A03762-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PARP3 at approximately 60 kDa. The expected band size for PARP3 is at 60 kDa.
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