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PARP3 Polyclonal Antibody

BS-7108R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityEquine, Human, Mouse, Rabbit, Rat
TargetPARP3
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Overview

  • Supplier
    Bioss
  • Product Name
    PARP3 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID10039
  • Target name
    PARP3
  • Target description
    poly(ADP-ribose) polymerase family member 3
  • Target synonyms
    ADPRT3, ADPRTL2, ADPRTL3, ARTD3, IRT1, PADPRT-3, protein mono-ADP-ribosyltransferase PARP3, ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase)-like 2, ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase)-like 3, ADP-ribosyltransferase diphtheria toxin-like 3, ADPRT-3, DNA ADP-ribosyltransferase PARP3, NAD(+) ADP-ribosyltransferase 3, NAD+ ADP-ribosyltransferase 3, poly [ADP-ribose] polymerase 3, poly(ADP-ribose) synthetase-3, poly[ADP-ribose] synthase 3, poly[ADP-ribose] synthetase 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Equine, Human, Mouse, Rabbit, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of PARP3 using anti-PARP3 antibody (A03762-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: monkey COS-7 whole cell lysates, Lane 2: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PARP3 antigen affinity purified polyclonal antibody (Catalog # A03762-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PARP3 at approximately 60 kDa. The expected band size for PARP3 is at 60 kDa.
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