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Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm.
Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm.
Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm.

Anti-PCBD1 Antibody

HPA061723
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetPCBD1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PCBD1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5092
  • Target name
    PCBD1
  • Target description
    pterin-4 alpha-carbinolamine dehydratase 1
  • Target synonyms
    4-alpha-hydroxy-tetrahydropterin dehydratase; 6-pyruvoyl-tetrahydropterin synthase/dimerization cofactor of hepatocyte nuclear factor 1 alpha (TCF1); DCOH; dimerizing cofactor for HNF1; PCBD; PCD; phenylalanine hydroxylase-stimulating protein; PHS; pterin-4 alpha-carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor 1 alpha; pterin-4-alpha-carbinolamine dehydratase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP61457
  • Protein Name
    Pterin-4-alpha-carbinolamine dehydratase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PCBD1 using anti-PCBD1 antibody (A07264-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCBD1 antigen affinity purified polyclonal antibody (Catalog # A07264-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCBD1 at approximately 12 kDa. The expected band size for PCBD1 is at 12 kDa.
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