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Immunohistochemical staining of human fallopian tube shows mdoerate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human fallopian tube shows mdoerate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human fallopian tube shows mdoerate cytoplasmic positivity in glandular cells.

Anti-PCBP2 Antibody

HPA038356
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetPCBP2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PCBP2
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5094
  • Target name
    PCBP2
  • Target description
    poly(rC) binding protein 2
  • Target synonyms
    alpha-CP2; epididymis secretory sperm binding protein; heterogeneous nuclear ribonucleoprotein E2; heterogenous nuclear ribonucleoprotein E2; hnRNP E2; HNRNPE2; hnRNP-E2; HNRPE2; poly(rC)-binding protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15366
  • Protein Name
    Poly(rC)-binding protein 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Western Blot Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate All lanes: PCBP2 antibody at 5.3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 39, 35, 36, 34 kDa Observed band size: 43 kDa
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Immunohistochemical staining of human cerebellum shows strong nuclear positivity in cells in molecular layer.
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Figure 1. Western blot analysis of PCBP2/hnRNP E2 using anti-PCBP2/hnRNP E2 antibody (A02425-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates, Lane 2: human HEPG2 whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCBP2/hnRNP E2 antigen affinity purified polyclonal antibody (Catalog # A02425-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCBP2/hnRNP E2 at approximately 39KD. The expected band size for PCBP2/hnRNP E2 is at 39KD.
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