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Western Blot Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate All lanes: PCBP2 antibody at 5.3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 39, 35, 36, 34 kDa Observed band size: 43 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate All lanes: PCBP2 antibody at 5.3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 39, 35, 36, 34 kDa Observed band size: 43 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate All lanes: PCBP2 antibody at 5.3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 39, 35, 36, 34 kDa Observed band size: 43 kDa

PCBP2 Antibody

CSB-PA622995LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPCBP2
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Overview

  • Supplier
    Cusabio
  • Product Name
    PCBP2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5094
  • Target name
    PCBP2
  • Target description
    poly(rC) binding protein 2
  • Target synonyms
    alpha-CP2; epididymis secretory sperm binding protein; heterogeneous nuclear ribonucleoprotein E2; heterogenous nuclear ribonucleoprotein E2; hnRNP E2; HNRNPE2; hnRNP-E2; HNRPE2; poly(rC)-binding protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15366
  • Protein Name
    Poly(rC)-binding protein 2
  • Scientific Description
    Single-stranded nucleic acid binding protein that binds preferentially to oligo dC. Major cellular poly(rC)-binding protein. Binds also poly(rU). Negatively regulates cellular antiviral responses mediated by MAVS signaling (PubMed:19881509). It acts as an adapter between MAVS and the E3 ubiquitin ligase ITCH, therefore triggering MAVS ubiquitination and degradation (PubMed:19881509).
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PCBP2/hnRNP E2 using anti-PCBP2/hnRNP E2 antibody (A02425-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates, Lane 2: human HEPG2 whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCBP2/hnRNP E2 antigen affinity purified polyclonal antibody (Catalog # A02425-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCBP2/hnRNP E2 at approximately 39KD. The expected band size for PCBP2/hnRNP E2 is at 39KD.
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