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Immunohistochemical staining of human kidney shows strong cytoplasmic and nuclear positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong cytoplasmic and nuclear positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong cytoplasmic and nuclear positivity in cells in tubules.

Anti-PCK1 Antibody

HPA006277
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetPCK1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PCK1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5105
  • Target name
    PCK1
  • Target description
    phosphoenolpyruvate carboxykinase 1
  • Target synonyms
    PCKDC; PEP carboxykinase; PEPCK1; PEPCKC; PEPCK-C; phosphoenolpyruvate carboxykinase 1 (soluble); phosphoenolpyruvate carboxykinase, cytosolic; phosphoenolpyruvate carboxykinase, cytosolic [GTP]; phosphoenolpyruvate carboxylase; phosphopyruvate carboxylase; serine-protein kinase PCK1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP35558
  • Protein Name
    Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Immunohistochemical staining of human kidney shows moderate cytoplasmic and nuclear positivity in renal tubules.
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Figure 1. Western blot analysis of PCK1/PEPC using anti-PCK1/PEPC antibody (A02022-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: rat kidney tissue lysates, Lane 4: mouse liver tissue lysates, Lane 5: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCK1/PEPC antigen affinity purified polyclonal antibody (Catalog # A02022-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCK1/PEPC at approximately 69KD. The expected band size for PCK1/PEPC is at 69KD.
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