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Western Blot analysis of HeLa MCF7 cells using PEPCK-C Polyclonal Antibody
Western Blot analysis of HeLa MCF7 cells using PEPCK-C Polyclonal Antibody
Western Blot analysis of HeLa MCF7 cells using PEPCK-C Polyclonal Antibody

PCK1 Antibody

CSB-PA005188
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetPCK1
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Overview

  • Supplier
    Cusabio
  • Product Name
    PCK1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5105
  • Target name
    PCK1
  • Target description
    phosphoenolpyruvate carboxykinase 1
  • Target synonyms
    PCKDC; PEP carboxykinase; PEPCK1; PEPCKC; PEPCK-C; phosphoenolpyruvate carboxykinase 1 (soluble); phosphoenolpyruvate carboxykinase, cytosolic; phosphoenolpyruvate carboxykinase, cytosolic [GTP]; phosphoenolpyruvate carboxylase; phosphopyruvate carboxylase; serine-protein kinase PCK1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP35558
  • Protein Name
    Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PCK1/PEPC using anti-PCK1/PEPC antibody (A02022-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: rat kidney tissue lysates, Lane 4: mouse liver tissue lysates, Lane 5: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCK1/PEPC antigen affinity purified polyclonal antibody (Catalog # A02022-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCK1/PEPC at approximately 69KD. The expected band size for PCK1/PEPC is at 69KD.
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