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Western blot analysis in human cell line U-2197.
Western blot analysis in human cell line U-2197.
Western blot analysis in human cell line U-2197.

Anti-PCOLCE Antibody

AMAB91432
Atlas Antibodies
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetPCOLCE
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PCOLCE
  • Delivery Days Customer
    4
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CL6567
  • Conjugate
    Unconjugated
  • Gene ID5118
  • Target name
    PCOLCE
  • Target description
    procollagen C-endopeptidase enhancer
  • Target synonyms
    PCPE; PCPE1; PCPE-1; procollagen C-endopeptidase enhancer 1; procollagen COOH-terminal proteinase enhancer 1; procollagen C-proteinase enhancer 1; procollagen, type 1, COOH-terminal proteinase enhancer; type 1 procollagen C-proteinase enhancer protein; type I procollagen COOH-terminal proteinase enhancer
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ15113
  • Protein Name
    Procollagen C-endopeptidase enhancer 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of PCOLCE using anti-PCOLCE antibody (A06428-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCOLCE antigen affinity purified polyclonal antibody (Catalog # A06428-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCOLCE at approximately 50 kDa. The expected band size for PCOLCE is at 48 kDa.
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