Anti-PCSK9 [508.20.33b (Fab33)], Mouse IgG2a, kappa

Absolute Antibody

Anti-PCSK9 [508.20.33b (Fab33)], Mouse IgG2a, kappa

9

This antibody binds human PCSK9. Proprotein convertase subtilisin/kexin type 9 (PCSK9) is a serine protease enzyme encoded by the PCSK9 gene in humans and it plays a role in regulation of circulating cholesterol. It is associated with autosomal dominant h

The binding affinity of this antibody towards PCSK9 protein from human, murine, rhesus, and cynomolgus monkey was done using surface plasmon resonance. This antibody was found to inhibit binding of PCSK9 to LDLR in a competition binding ELISA. This antibody plays a role in effective prevention of LDLR downregulation in HepG2 cells in vitro. When administered in vivo, this antibody prevented LDLR downregulation in mouse liver. This antibody showed a reduction in total serum cholesterol levels in mice when a single dose of 10 mg/kg was administered. It was also found that a combination of this antibody and statin results in a greater reduction in total cholesterol level compared to anti-PCSK9 antibody alone or statin alone treatments (US9266961). A randomized, double-blind, placebo-controlled, single and multiple dose study was conducted to evaluate, primarily, the safety and tolerability of single and multiple (four weekly) doses of reformatted IgG1 version of this antibody when administered by subcutaneous (SC) injection to healthy volunteers with elevated serum low-density lipoprotein cholesterol (LDL-c) concentration. It was found that there were no serious or severe adverse events, no discontinuations for adverse events, and no dose-limiting toxicities (US9266961).

Functional Assay, ELISA, Neutralisation/Blocking, Other Application

Research Use Only

Monoclonal

508.20.33b (Fab33)

PCSK9

proprotein convertase subtilisin/kexin type 9

convertase subtilisin/kexin type 9 preproprotein; FH3; FHCL3; HCHOLA3; LDLCQ1; NARC1; NARC-1; neural apoptosis regulated convertase 1; PC9; proprotein convertase subtilisin/kexin type 9; subtilisin/kexin-like protease PC9

Mouse

IgG2a

Proprotein convertase subtilisin/kexin type 9

This is a chimeric mouse IgG2a antibody made using the variable domain sequences of the original human IgG1 format. It was created to improve compatibility with existing reagents, assays, and techniques.

Human, Monkey, Mouse, Primate, Rat

-20°C,2°C to 8°C

12352203