Anti-PCSK9 [6E2]

Absolute Antibody

Anti-PCSK9 [6E2]

7

This antibody binds a helical conformational epitope formed by the acidic amino acid stretch comprising amino acids between Q31-A53 of the human PCSK9, particularly the segment DYEELVLAL. It can bind intact human PCSK9 but can not bind mouse PCSK9. Propro

Proprotein convertase subtilisin/kexin type 9 (PCSK9) regulates serum LDL cholesterol (LDL-C) by interacting with the LDL receptor (LDLR) and is an attractive therapeutic target for LDL-C lowering. The epitope mapping of this antibody was done using western blotting. This antibody was able to identify a 16 kDa prodomain of recombinant PCSK9 but not the 60 kDa band comprising the catalytic and C-terminal domain in western blot. Surface plasmon resonance demonstrated that Ab6E2 (IgG1) bound to PCSK9 with a KD of 10.5 +/- 1.2 nM. Biolayer interferometry experiments suggested that the formation of proper alpha-helix is required for the binding interaction of this antibody. This antibody was also used for immunoprecipitation of secreted native PCSK9 from HepG2, IM-95m and H2073 cell l ins and of recombinant PCSK9 (PMID: 30653992).

ImmunoPrecipitation, Western Blot, Other Application

WB; SPR; BLI; IP

Research Use Only

Monoclonal

6E2

PCSK9

proprotein convertase subtilisin/kexin type 9

convertase subtilisin/kexin type 9 preproprotein; FH3; FHCL3; HCHOLA3; LDLCQ1; NARC1; NARC-1; neural apoptosis regulated convertase 1; PC9; proprotein convertase subtilisin/kexin type 9; subtilisin/kexin-like protease PC9

Rabbit

IgG

Proprotein convertase subtilisin/kexin type 9

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

Human

Human

The original antibody was generated by immunization of PCSK9 knock-out mice with recombinant human PCSK9.

-20°C,2°C to 8°C

12352203