Anti-PCSK9 [6E2], Human IgG1, Fc Silent(tm), kappa

Absolute Antibody

Anti-PCSK9 [6E2], Human IgG1, Fc Silent(tm), kappa

9

This antibody binds a helical conformational epitope formed by the acidic amino acid stretch comprising amino acids between Q31-A53 of the human PCSK9, particularly the segment DYEELVLAL. It can bind intact human PCSK9 but can not bind mouse PCSK9. Propro

Proprotein convertase subtilisin/kexin type 9 (PCSK9) regulates serum LDL cholesterol (LDL-C) by interacting with the LDL receptor (LDLR) and is an attractive therapeutic target for LDL-C lowering. The epitope mapping of this antibody was done using western blotting. This antibody was able to identify a 16 kDa prodomain of recombinant PCSK9 but not the 60 kDa band comprising the catalytic and C-terminal domain in western blot. Surface plasmon resonance demonstrated that Ab6E2 (IgG1) bound to PCSK9 with a KD of 10.5 +/- 1.2 nM. Biolayer interferometry experiments suggested that the formation of proper alpha-helix is required for the binding interaction of this antibody. This antibody was also used for immunoprecipitation of secreted native PCSK9 from HepG2, IM-95m and H2073 cell l ins and of recombinant PCSK9 (PMID: 30653992).

ImmunoPrecipitation, Western Blot, Other Application

Research Use Only

Monoclonal

6E2

PCSK9

proprotein convertase subtilisin/kexin type 9

convertase subtilisin/kexin type 9 preproprotein; FH3; FHCL3; HCHOLA3; LDLCQ1; NARC1; NARC-1; neural apoptosis regulated convertase 1; PC9; proprotein convertase subtilisin/kexin type 9; subtilisin/kexin-like protease PC9

Human

IgG1

Proprotein convertase subtilisin/kexin type 9

This is a reformatted human IgG1 Fc Silent™ antibody, based on the original human IgG1 format, created for improved compatibility with existing reagents, assays and techniques.

Human

-20°C,2°C to 8°C

12352203