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Anti-PCSK9 Reference Antibody (Merck patent anti-PCSK9)

M00085-16
Boster Bio
ApplicationsFunctional Assay, Flow Cytometry, ELISA, Other Application
Product group Antibodies
ReactivityHuman, Mouse
TargetPCSK9

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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-PCSK9 Reference Antibody (Merck patent anti-PCSK9)
  • Delivery Days Customer
    23
  • Applications
    Functional Assay, Flow Cytometry, ELISA, Other Application
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Concentration
    1 mg/ml
  • Gene ID255738
  • Target name
    PCSK9
  • Target description
    proprotein convertase subtilisin/kexin type 9
  • Target synonyms
    FH3, FHCL3, HCHOLA3, LDLCQ1, NARC-1, NARC1, PC9, proprotein convertase subtilisin/kexin type 9, convertase subtilisin/kexin type 9 preproprotein, neural apoptosis regulated convertase 1, subtilisin/kexin-like protease PC9
  • Isotype
    IgG1
  • Protein IDQ8NBP7
  • Protein Name
    Proprotein convertase subtilisin/kexin type 9
  • Scientific Description
    Boster Bio Anti-PCSK9 Reference Antibody (Merck patent anti-PCSK9) (Catalog # M00085-16). Tested in Flow Cytometry, ELISA, FTA. This antibody reacts with Human, Mouse. Endotoxin: < 0.167EU/microg,determined by LAL method. Expression system: CHO Cell
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PCSK9 using anti-PCSK9 antibody (A00085-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human CACO-2 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCSK9 antigen affinity purified polyclonal antibody (Catalog # A00085-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCSK9 at approximately 66 kDa, 80 kDa (precursor). The expected band size for PCSK9 is at 72 kDa.
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