Anti-PD-L2 [24F.10C12]

Absolute Antibody

Anti-PD-L2 [24F.10C12]

9

This antibody binds human PD-L2, expressed on dendritic cells, IFN-gamma-treated monocytes, placental endothelium and medullary thymic epithelial cells.

This antibody can recognize PD-L2-Fc in an ELISA. Flow cytometric analysis with whole anti-PD-L2 mAb 24F.10C12 and its Fab version revealed approximately the same mean fluorescence intensity upon binding PD-L2 transfected cells. This antibody is capable of blocking the binding of PD-1-Ig to PD-L2 in a dose-dependent manner and with high avidity. This antibody was also used for the immunohistochemical studies of various tissues including thymus, germinal center, placenta, cardiac tissues, and tumors. Blockade of PD-L2 on dendritic cells results in enhanced T cell proliferation and cytokine production, including that of IFN-gamma and IL-10. Both the whole antibody version and Fab version enhanced T cell activation, showing that PD-L2 functions to inhibit T cell activation (PMID: 12538684). This antibody was used for the study of PD-1 pathway on the activation of naive, memory, and recently activated human CD4+ T cells (PMID: 15598424). Antibody blockade of endothelial cell PD-L2 enhanced endothelial cell costimulation of PHA-activated human CD8+ T cells (PMID: 14579280).

Flow Cytometry, ELISA, ImmunoHistoChemistry, Neutralisation/Blocking

Research Use Only

Monoclonal

24F.10C12

PDCD1LG2

programmed cell death 1 ligand 2

B7 dendritic cell molecule; B7DC; B7-DC; bA574F11.2; Btdc; butyrophilin B7-DC; CD273; PD-1-ligand 2; PDCD1 ligand 2; PDCD1L2; PDL2; PD-L2; programmed cell death 1 ligand 2; programmed death ligand 2

Mouse

IgG2a

Programmed cell death 1 ligand 2

Human

-20°C,2°C to 8°C

12352203