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Anti-PD-L2 [366C.9E5]

Ab01479-10.0
Absolute Antibody
ApplicationsImmunoHistoChemistry
Product group Antibodies
TargetPDCD1LG2
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-PD-L2 [366C.9E5]
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody has been used in the immunohistochemical detection of PD-L2 in formalin-fixed, paraffin-embedded tissue sections of Hodgkins lymphoma tissue sections (Ansell et al, 2018; Roemer et al, 2016), primary mediastinal large B-cell lymphoma and diffuse large B-cell lymphoma biopsies (Shi et al, 2014; Chong et al, 2016), non-small-cell lung cancer tumour samples (Calles et al, 2015), primary and metastatic adenoid cystic carcinoma deposits (Sridharan et al, 2016), and tonsil and colorectal carcinoma tissue samples (Masugi et al, 2017).
  • Applications
    ImmunoHistoChemistry
  • Applications Supplier
    IHC
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    366C.9E5
  • Gene ID80380
  • Target name
    PDCD1LG2
  • Target description
    programmed cell death 1 ligand 2
  • Target synonyms
    B7 dendritic cell molecule; B7DC; B7-DC; bA574F11.2; Btdc; butyrophilin B7-DC; CD273; PD-1-ligand 2; PDCD1 ligand 2; PDCD1L2; PDL2; PD-L2; programmed cell death 1 ligand 2; programmed death ligand 2
  • Host
    Human
  • Isotype
    IgG1
  • Protein IDQ9BQ51
  • Protein Name
    Programmed cell death 1 ligand 2
  • Scientific Description
    This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    This antibody was raised by immunising mice with a recombinant protein fragment corresponding to the extracellular domain of human PD-L2 (as an Ig fusion protein).
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of B7 DC using anti-B7 DC antibody (PB10010). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: HELA whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-B7 DC antigen affinity purified polyclonal antibody (Catalog # PB10010) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for B7 DC at approximately 37 kDa. The expected band size for B7 DC is at 31 kDa.
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