Figure 1. Western blot analysis of PDE6 beta/PDE6B using anti-PDE6 beta/PDE6B antibody (M02659-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat eye tissue lysates, Lane 2: mouse eye tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-PDE6 beta/PDE6B antigen affinity purified monoclonal antibody (Catalog # M02659-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for PDE6 beta/PDE6B at approximately 98 kDa. The expected band size for PDE6 beta/PDE6B is at 98 kDa.
Figure 1. Western blot analysis of PDE6 beta/PDE6B using anti-PDE6 beta/PDE6B antibody (M02659-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat eye tissue lysates, Lane 2: mouse eye tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-PDE6 beta/PDE6B antigen affinity purified monoclonal antibody (Catalog # M02659-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for PDE6 beta/PDE6B at approximately 98 kDa. The expected band size for PDE6 beta/PDE6B is at 98 kDa.
Anti-PDE6 beta/PDE6B Antibody Picoband(r) (monoclonal, 8I2D7)
M02659-1-IFLUOR647
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPDE6B
Overview
- SupplierBoster Bio
- Product NameAnti-PDE6 beta/PDE6B Antibody Picoband(r) (monoclonal, 8I2D7)
- Delivery Days Customer9
- Application Supplier NoteTested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID8I2D7
- Concentration500 ug/ml
- ConjugateOther Conjugate
- Gene ID5158
- Target namePDE6B
- Target descriptionphosphodiesterase 6B
- Target synonymsCSNB3; CSNBAD2; GMP-PDE beta; GMP-PDEbeta; PDEB; phosphodiesterase 6B, cGMP-specific, rod, beta; rd1; rod cGMP-phosphodiesterase beta-subunit; rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta; RP40
- HostMouse
- IsotypeIgG2a
- Protein IDP35913
- Protein NameRod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta
- Scientific DescriptionBoster Bio Anti-PDE6 beta/PDE6B Antibody Picoband® (monoclonal, 8I2D7) catalog # M02659-1. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203