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Immunohistochemical staining of human retina shows strong positivity in photoreceptor cells.
Immunohistochemical staining of human retina shows strong positivity in photoreceptor cells.
Immunohistochemical staining of human retina shows strong positivity in photoreceptor cells.

Anti-PDE6B Antibody

HPA054148
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPDE6B
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PDE6B
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5158
  • Target name
    PDE6B
  • Target description
    phosphodiesterase 6B
  • Target synonyms
    CSNB3, CSNBAD2, GMP-PDEbeta, PDEB, RP40, rd1, rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta, GMP-PDE beta, phosphodiesterase 6B, cGMP-specific, rod, beta, rod cGMP-phosphodiesterase beta-subunit
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP35913
  • Protein Name
    Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of PDE6 beta/PDE6B using anti-PDE6 beta/PDE6B antibody (A02659-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat eye tissue lysates, Lane 2: mouse eye tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDE6 beta/PDE6B antigen affinity purified polyclonal antibody (Catalog # A02659-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDE6 beta/PDE6B at approximately 98KD. The expected band size for PDE6 beta/PDE6B is at 98KD.
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