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Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.

Anti-PDIA4 Antibody

HPA006139
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPDIA4
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PDIA4
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9601
  • Target name
    PDIA4
  • Target description
    protein disulfide isomerase family A member 4
  • Target synonyms
    ERP70, ERP72, ERp-72, protein disulfide-isomerase A4, ER protein 70, ER protein 72, endoplasmic reticulum resident protein 70, endoplasmic reticulum resident protein 72, protein disulfide isomerase related protein (calcium-binding protein, intestinal-related), protein disulfide isomerase-associated 4, testicular tissue protein Li 137
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP13667
  • Protein Name
    Protein disulfide-isomerase A4
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of ERp72/PDIA4 using anti-ERp72/PDIA4 antibody (A07267-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human PANC-1 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERp72/PDIA4 antigen affinity purified polyclonal antibody (Catalog # A07267-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERp72/PDIA4 at approximately 72 kDa. The expected band size for ERp72/PDIA4 is at 72 kDa.
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