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Western Blot Positive WB detected in: Mouse brain tissue, Mouse heart tissue, Mouse kidney tissue All lanes: PDIA4 antibody at 4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 73 kDa Observed band size: 73 kDa
Western Blot Positive WB detected in: Mouse brain tissue, Mouse heart tissue, Mouse kidney tissue All lanes: PDIA4 antibody at 4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 73 kDa Observed band size: 73 kDa
Western Blot Positive WB detected in: Mouse brain tissue, Mouse heart tissue, Mouse kidney tissue All lanes: PDIA4 antibody at 4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 73 kDa Observed band size: 73 kDa

PDIA4 Antibody

CSB-PA017722LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetPDIA4
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Overview

  • Supplier
    Cusabio
  • Product Name
    PDIA4 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9601
  • Target name
    PDIA4
  • Target description
    protein disulfide isomerase family A member 4
  • Target synonyms
    endoplasmic reticulum resident protein 70; endoplasmic reticulum resident protein 72; ER protein 70; ER protein 72; ERP70; ERP72; ERp-72; protein disulfide isomerase related protein (calcium-binding protein, intestinal-related); protein disulfide isomerase-associated 4; protein disulfide-isomerase A4; testicular tissue protein Li 137
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP13667
  • Protein Name
    Protein disulfide-isomerase A4
  • Scientific Description
    cell surface, endoplasmic reticulum lumen, poly(A) RNA binding, protein disulfide isomerase activity, chaperone-mediated protein folding, protein folding, protein secretion, response to endoplasmic reticulum stress
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ERp72/PDIA4 using anti-ERp72/PDIA4 antibody (A07267-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human PANC-1 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERp72/PDIA4 antigen affinity purified polyclonal antibody (Catalog # A07267-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERp72/PDIA4 at approximately 72 kDa. The expected band size for ERp72/PDIA4 is at 72 kDa.
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