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Immunohistochemical staining of human small intestine shows weak to moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human small intestine shows weak to moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human small intestine shows weak to moderate cytoplasmic positivity in glandular cells.

Anti-PDLIM4-25ul

HPA011912
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Rat
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PDLIM4-25ul
  • Delivery Days Customer
    6
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    IHC, ICC, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.1
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP50479
  • Protein Name
    PDZ and LIM domain protein 4
  • Scientific Description
    Rabbit Polyclonal Anti-PDLIM4 Antibody against Human PDZ and LIM domain 4. Validated for Immunofluorescence, Immunohistochemistry and Western Blot
  • Reactivity
    Human, Rat
  • Storage Instruction
    Store at +4°C for short term storage. Long time storage is recommended at -20°C.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PDLIM4 using anti-PDLIM4 antibody (A09573-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hacat whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human U20S whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDLIM4 antigen affinity purified polyclonal antibody (Catalog # A09573-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDLIM4 at approximately 35 kDa. The expected band size for PDLIM4 is at 35,26 kDa.
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