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Immunohistochemical staining of human Testis shows strong nuclear positivity in cells in seminiferous ducts and Leydig cells.
Immunohistochemical staining of human Testis shows strong nuclear positivity in cells in seminiferous ducts and Leydig cells.
Immunohistochemical staining of human Testis shows strong nuclear positivity in cells in seminiferous ducts and Leydig cells.

Anti-PDS5B Antibody

HPA040015
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPDS5B
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PDS5B Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23047
  • Target name
    PDS5B
  • Target description
    PDS5 cohesin associated factor B
  • Target synonyms
    APRIN, AS3, CG008, sister chromatid cohesion protein PDS5 homolog B, androgen induced inhibitor of proliferation, androgen-induced proliferation inhibitor, androgen-induced prostate proliferative shutoff-associated protein AS3, androgen-induced shutoff 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NTI5
  • Protein Name
    Sister chromatid cohesion protein PDS5 homolog B
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PDS5B using anti-PDS5B antibody (A04823-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human Daudi whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDS5B antigen affinity purified polyclonal antibody (Catalog # A04823-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDS5B at approximately 170 kDa. The expected band size for PDS5B is at 165 kDa.
Product group Antibodies
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ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman, Mouse
TargetPDS5B
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Protein IDQ9NTI5
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Mouse brain lysates probed with PDS5B Polyclonal Antibody, Unconjugated (bs-12605R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
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ReactivityBovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat, Sheep
TargetPDS5B
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Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane: Hela cells, Primary antibody: CSB-PA243039(PDS5B Antibody) at dilution 1/316.6, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds
Product group Antibodies
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Immunohistochemical staining of human kidney shows strong nuclear positivity in cells in tubules and cells in glomeruli.
Product group Antibodies
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Sample (50 ug of whole cell lysate) A: Mouse brain 5% SDS PAGE GTX116345 diluted at 1:1000
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