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Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane: Hela cells, Primary antibody: CSB-PA243039(PDS5B Antibody) at dilution 1/316.6, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane: Hela cells, Primary antibody: CSB-PA243039(PDS5B Antibody) at dilution 1/316.6, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane: Hela cells, Primary antibody: CSB-PA243039(PDS5B Antibody) at dilution 1/316.6, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds

PDS5B Antibody

CSB-PA243039
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPDS5B
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Overview

  • Supplier
    Cusabio
  • Product Name
    PDS5B Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23047
  • Target name
    PDS5B
  • Target description
    PDS5 cohesin associated factor B
  • Target synonyms
    APRIN, AS3, CG008, sister chromatid cohesion protein PDS5 homolog B, androgen induced inhibitor of proliferation, androgen-induced proliferation inhibitor, androgen-induced prostate proliferative shutoff-associated protein AS3, androgen-induced shutoff 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NTI5
  • Protein Name
    Sister chromatid cohesion protein PDS5 homolog B
  • Scientific Description
    This gene encodes a protein that interacts with the conserved protein complex termed cohesion. The cohesion complex holds together sister chromatids and facilitates accurate chromosome segregation during mitosis and meiosis. This protein is also a negative regulator of cell proliferation and may be a tumor-suppressor gene.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PDS5B using anti-PDS5B antibody (A04823-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human Daudi whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDS5B antigen affinity purified polyclonal antibody (Catalog # A04823-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDS5B at approximately 170 kDa. The expected band size for PDS5B is at 165 kDa.
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