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Immunohistochemical staining of human kidney shows strong cytoplasmic and membranous positivity in tubular cells.
Immunohistochemical staining of human kidney shows strong cytoplasmic and membranous positivity in tubular cells.
Immunohistochemical staining of human kidney shows strong cytoplasmic and membranous positivity in tubular cells.

Anti-PDXK Antibody

HPA030198
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetPDXK
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PDXK
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8566
  • Target name
    PDXK
  • Target description
    pyridoxal kinase
  • Target synonyms
    C21orf124; C21orf97; epididymis secretory sperm binding protein Li 1a; HEL-S-1a; HMSN6C; PKH; PNK; PRED79; pyridoxal (pyridoxine, vitamin B6) kinase; pyridoxal kinase; pyridoxamine kinase; pyridoxine kinase; vitamin B6 kinase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00764
  • Protein Name
    Pyridoxal kinase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Western blot All lanes: PDXK antibody at 5.63microg/ml Lane 1: Mouse liver tissue Lane 2: Hela whole cell lysate Lane 3: Mouse kidney tissue Lane 4: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 36, 32, 27 kDa Observed band size: 36 kDa
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Figure 1. Western blot analysis of PDXK using anti-PDXK antibody (A06683-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human SiHa whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDXK antigen affinity purified polyclonal antibody (Catalog # A06683-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDXK at approximately 35 kDa. The expected band size for PDXK is at 35,40 kDa.
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