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Immunohistochemical staining of human hippocampus shows moderate to strong cytoplasmic positivity in neurons.
Immunohistochemical staining of human hippocampus shows moderate to strong cytoplasmic positivity in neurons.
Immunohistochemical staining of human hippocampus shows moderate to strong cytoplasmic positivity in neurons.

Anti-PDXP Antibody

HPA000531
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPDXP
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PDXP
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID57026
  • Target name
    PDXP
  • Target description
    pyridoxal phosphatase
  • Target synonyms
    chronophin; CIN; dJ37E16.5; PLP; PLP phosphatase; pyridoxal (pyridoxine, vitamin B6) phosphatase; pyridoxal phosphate phosphatase; testicular secretory protein Li 36
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96GD0
  • Protein Name
    Pyridoxal phosphate phosphatase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of PDXP using anti-PDXP antibody (A09629-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDXP antigen affinity purified polyclonal antibody (Catalog # A09629-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDXP at approximately 37 kDa. The expected band size for PDXP is at 32 kDa.
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