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PDXP / Pyridoxal Phosphatase Antibody

LS-C672908
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetPDXP
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PDXP / Pyridoxal Phosphatase Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID57026
  • Target name
    PDXP
  • Target description
    pyridoxal phosphatase
  • Target synonyms
    CIN, PLP, dJ37E16.5, chronophin, PLP phosphatase, pyridoxal (pyridoxine, vitamin B6) phosphatase, pyridoxal phosphate phosphatase, testicular secretory protein Li 36
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PDXP using anti-PDXP antibody (A09629-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDXP antigen affinity purified polyclonal antibody (Catalog # A09629-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDXP at approximately 37 kDa. The expected band size for PDXP is at 32 kDa.
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