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Immunohistochemical staining of human endometrium shows moderate to strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human endometrium shows moderate to strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human endometrium shows moderate to strong cytoplasmic positivity in glandular cells.

Anti-PER3 Antibody

HPA019530
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPER3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PER3
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8863
  • Target name
    PER3
  • Target description
    period circadian regulator 3
  • Target synonyms
    FASPS3, GIG13, period circadian protein homolog 3, cell growth-inhibiting gene 13 protein, circadian clock protein PERIOD 3, period circadian clock 3, period circadian protein 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP56645
  • Protein Name
    Period circadian protein homolog 3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PER3 using anti-PER3 antibody (A01835-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela cell lysate, Lane 2: human MCF-7 cell lysate, Lane 3: human A549 cell lysate, Lane 4: human HepG2 cell lysate, Lane 5: human COLO-320 cell lysate, Lane 6: rat heart tissue lysate, Lane 7: mouse skeletal muscle tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PER3 antigen affinity purified polyclonal antibody (Catalog # A01835-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PER3 at approximately 160KD. The expected band size for PER3 is at 132KD.
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