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WB analysis of Jurkat cells treated with insulin 0.01U/ml (15mins) lysate using GTX87243 PER3 antibody. The lane on the right is blocked with the synthesized peptide.
WB analysis of Jurkat cells treated with insulin 0.01U/ml (15mins) lysate using GTX87243 PER3 antibody. The lane on the right is blocked with the synthesized peptide.
WB analysis of Jurkat cells treated with insulin 0.01U/ml (15mins) lysate using GTX87243 PER3 antibody. The lane on the right is blocked with the synthesized peptide.

PER3 antibody

GTX87243
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetPER3
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Overview

  • Supplier
    GeneTex
  • Product Name
    PER3 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500~1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8863
  • Target name
    PER3
  • Target description
    period circadian regulator 3
  • Target synonyms
    cell growth-inhibiting gene 13 protein; circadian clock protein PERIOD 3; FASPS3; GIG13; period circadian clock 3; period circadian protein 3; period circadian protein homolog 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP56645
  • Protein Name
    Period circadian protein homolog 3
  • Scientific Description
    This gene is a member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene have been linked to sleep disorders. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2014]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PER3 using anti-PER3 antibody (A01835-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela cell lysate, Lane 2: human MCF-7 cell lysate, Lane 3: human A549 cell lysate, Lane 4: human HepG2 cell lysate, Lane 5: human COLO-320 cell lysate, Lane 6: rat heart tissue lysate, Lane 7: mouse skeletal muscle tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PER3 antigen affinity purified polyclonal antibody (Catalog # A01835-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PER3 at approximately 160KD. The expected band size for PER3 is at 132KD.
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