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Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.

Anti-PEX19 Antibody

HPA044837
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPEX19
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PEX19
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5824
  • Target name
    PEX19
  • Target description
    peroxisomal biogenesis factor 19
  • Target synonyms
    D1S2223E, HK33, PBD12A, PMP1, PMPI, PXF, PXMP1, peroxisomal biogenesis factor 19, 33 kDa housekeeping protein, housekeeping gene, 33kD, peroxin-19, peroxisomal farnesylated protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP40855
  • Protein Name
    Peroxisomal biogenesis factor 19
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of PEX19 using anti-PEX19 antibody (A03186-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human SiHa whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PEX19 antigen affinity purified polyclonal antibody (Catalog # A03186-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PEX19 at approximately 38 kDa. The expected band size for PEX19 is at 33 kDa.
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