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Immunohistochemistry of paraffin-embedded human small intestine tissue using CSB-PA017802DSR2HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human small intestine tissue using CSB-PA017802DSR2HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human small intestine tissue using CSB-PA017802DSR2HU at dilution of 1:100

PEX19 Antibody

CSB-PA017802DSR2HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPEX19
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Overview

  • Supplier
    Cusabio
  • Product Name
    PEX19 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5824
  • Target name
    PEX19
  • Target description
    peroxisomal biogenesis factor 19
  • Target synonyms
    33 kDa housekeeping protein; D1S2223E; HK33; housekeeping gene, 33kD; PBD12A; peroxin-19; peroxisomal biogenesis factor 19; peroxisomal farnesylated protein; PMP1; PMPI; PXF; PXMP1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP40855
  • Protein Name
    Peroxisomal biogenesis factor 19
  • Scientific Description
    Necessary for early peroxisomal biogenesis. Acts both as a cytosolic chaperone and as an import receptor for peroxisomal membrane proteins (PMPs). Binds and stabilizes newly synthesized PMPs in the cytoplasm by interacting with their hydrophobic membrane-spanning domains, and targets them to the peroxisome membrane by binding to the integral membrane protein PEX3. Excludes CDKN2A from the nucleus and prevents its interaction with MDM2, which results in active degradation of TP53.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PEX19 using anti-PEX19 antibody (A03186-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human SiHa whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PEX19 antigen affinity purified polyclonal antibody (Catalog # A03186-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PEX19 at approximately 38 kDa. The expected band size for PEX19 is at 33 kDa.
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