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Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in megakaryocytes.
Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in megakaryocytes.
Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in megakaryocytes.

Anti-PF4 Antibody

HPA052485
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetPF4
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PF4
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5196
  • Target name
    PF4
  • Target description
    platelet factor 4
  • Target synonyms
    chemokine (C-X-C motif) ligand 4; C-X-C motif chemokine 4; CXCL4; iroplact; oncostatin-A; PF-4; platelet factor 4; SCYB4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP02776
  • Protein Name
    Platelet factor 4
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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IHC image of CSB-PA017809LA01HU diluted at 1:200 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Figure 1. Western blot analysis of PF4 using anti-PF4 antibody (PB10011). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: 293T whole cell lysates, Lane 2: A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PF4 antigen affinity purified polyclonal antibody (Catalog # PB10011) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PF4 at approximately 26 kDa. The expected band size for PF4 is at 11 kDa.
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IHC-P analysis of rat bone marrow section using PF4 antibody [GT1260] PF4 antibody [GT1260]. Dilution : 1:100 Blue : DAPI for nuclear staining.
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