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Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in megakaryocytes.
Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in megakaryocytes.
Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in megakaryocytes.

Anti-PF4 Antibody

HPA052485
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPF4
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PF4
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5196
  • Target name
    PF4
  • Target description
    platelet factor 4
  • Target synonyms
    CXCL4, PF-4, SCYB4, platelet factor 4, C-X-C motif chemokine 4, chemokine (C-X-C motif) ligand 4, iroplact, oncostatin-A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP02776
  • Protein Name
    Platelet factor 4
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PF4 using anti-PF4 antibody (PB10011). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: 293T whole cell lysates, Lane 2: A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PF4 antigen affinity purified polyclonal antibody (Catalog # PB10011) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PF4 at approximately 26 kDa. The expected band size for PF4 is at 11 kDa.
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