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Rabbit anti Human platelet factor 4

RAHu/PF4
Nordic-MUbio
TargetPF4
Product group Antibodies
1 ml
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Rabbit anti Human platelet factor 4
  • Delivery Days Customer
    7
  • Application Supplier Note
    Precipitation assays. In immunoelectrophoresis use 2 microl serum or equivalent against 120 microl antiserum. In double radial immunodiffusion use a rosette arrangement with 10 microl antiserum in 3 mm diameter centre well and 2 microl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells. In electroimmunodiffusion the amount of antiserum required in the agarose gel is usually between 1 and 2%, depending on the test arrangement. The amount of platelet factor 4 precipitated by 1 ml antiserum is approximately 0.1 mg.
  • Category Supplier
    Primary antibodies
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID5196
  • Target name
    PF4
  • Target description
    platelet factor 4
  • Target synonyms
    chemokine (C-X-C motif) ligand 4; C-X-C motif chemokine 4; CXCL4; iroplact; oncostatin-A; PF-4; platelet factor 4; SCYB4
  • Host
    Rabbit
  • Protein IDP02776
  • Protein Name
    Platelet factor 4
  • Source
    Platelet factor 4 is a low molecular weight (MW 7,800) heat stable protein, secreted by alpha granules of the platelets. Purified platelet factor 4 isolated from pooled human serum. Freunds complete adjuvant is used in the first step of the ...
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PF4 using anti-PF4 antibody (PB10011). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: 293T whole cell lysates, Lane 2: A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PF4 antigen affinity purified polyclonal antibody (Catalog # PB10011) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PF4 at approximately 26 kDa. The expected band size for PF4 is at 11 kDa.
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